| Speaker | Play | Sex | Lines |
| ROSALIND | As You Like It | Female | 685 |
| CLEOPATRA | Antony and Cleopatra | Female | 678 |
| INNOGEN | Cymbeline | Female | 594 |
| PORTIA | The Merchant of Venice | Female | 574 |
| JULIET | Romeo and Juliet | Female | 542 |
| HELENA | All's Well That Ends Well | Female | 478 |
| ISABELLA | Measure for Measure | Female | 424 |
| DESDEMONA | Othello | Female | 391 |
| EMILIA | The Two Noble Kinsmen | Female | 374 |
| VIOLA | Twelfth Night | Female | 335 |
| PAULINA | The Winter's Tale | Female | 332 |
| MISTRESS PAGE | The Merry Wives of Windsor | Female | 328 |
| DAUGHTER | The Two Noble Kinsmen | Female | 324 |
| JULIA | The Two Gentlemen of Verona | Female | 322 |
| QUEEN | Henry VI Part 2 | Female | 316 |
| OLIVIA | Twelfth Night | Female | 314 |
| VOLUMNIA | Coriolanus | Female | 311 |
| CRESSIDA | Troilus and Cressida | Female | 301 |
| COUNTESS | All's Well That Ends Well | Female | 291 |
| PRINCESS | Love's Labour's Lost | Female | 288 |
| CELIA | As You Like It | Female | 282 |
| QUEEN | Henry VI Part 3 | Female | 279 |
| BEATRICE | Much Ado About Nothing | Female | 279 |
| NURSE | Romeo and Juliet | Female | 277 |
| QUEEN ELIZABETH | Richard III | Female | 272 |
| CONSTANCE | King John | Female | 264 |
| ADRIANA | The Comedy of Errors | Female | 263 |
| QUEEN KATHERINE | Henry VIII | Female | 261 |
| LADY | Macbeth | Female | 259 |
| TAMORA | Titus Andronicus | Female | 257 |
| PUCELLE | Henry VI Part 1 | Female | 254 |
| EMILIA | Othello | Female | 243 |
| MISTRESS QUICKLY | The Merry Wives of Windsor | Female | 236 |
| HELENA | A Midsummer Night's Dream | Female | 230 |
| KATHERINA | The Taming of the Shrew | Female | 221 |
| QUEEN MARGARET | Richard III | Female | 218 |
| COUNTESS | King Edward III | Female | 214 |
| HERMIONE | The Winter's Tale | Female | 211 |
| GONERILL | King Lear | Female | 199 |
| MISTRESS FORD | The Merry Wives of Windsor | Female | 192 |
| REGAN | King Lear | Female | 190 |
| MARINA | Pericles | Female | 190 |
| OPHELIA | Hamlet | Female | 173 |
| HOSTESS | Henry IV Part 2 | Female | 171 |
| QUEEN | Cymbeline | Female | 168 |
| ROSALINE | Love's Labour's Lost | Female | 165 |
| HERMIA | A Midsummer Night's Dream | Female | 164 |
| QUEEN | Hamlet | Female | 157 |
| ANNE | Richard III | Female | 157 |
| MIRANDA | The Tempest | Female | 153 |
| MARIA | Twelfth Night | Female | 149 |
| DUCHESS OF YORK | Richard III | Female | 141 |
| TITANIA | A Midsummer Night's Dream | Female | 141 |
| DIANA | All's Well That Ends Well | Female | 138 |
| HERO | Much Ado About Nothing | Female | 132 |
| PERDITA | The Winter's Tale | Female | 126 |
| DUCHESS | Henry VI Part 2 | Female | 119 |
| CORDELIA | King Lear | Female | 117 |
| LADY CAPULET | Romeo and Juliet | Female | 115 |
| QUEEN ISABEL | Richard II | Female | 115 |
| KATHERINE | Henry VIII | Female | 115 |
| CHARMIAN | Antony and Cleopatra | Female | 109 |
| BAWD | Pericles | Female | 104 |
| HIPPOLYTA | The Two Noble Kinsmen | Female | 102 |
| LUCIANA | The Comedy of Errors | Female | 96 |
| DUCHESS OF YORK | Richard II | Female | 93 |
| PORTIA | Julius Caesar | Female | 92 |
| NERISSA | The Merchant of Venice | Female | 88 |
| DIONYZA | Pericles | Female | 88 |
| JESSICA | The Merchant of Venice | Female | 86 |
| PHEBE | As You Like It | Female | 86 |
| THAISA | Pericles | Female | 80 |
| DOLL | Henry IV Part 2 | Female | 78 |
| LADY GREY | Henry VI Part 3 | Female | 73 |
| ABBESS | The Comedy of Errors | Female | 73 |
| LUCETTA | The Two Gentlemen of Verona | Female | 72 |
| BIANCA | The Taming of the Shrew | Female | 69 |
| MARIANA | Measure for Measure | Female | 68 |
| MARGARET | Much Ado About Nothing | Female | 68 |
| OLD LADY | Henry VIII | Female | 68 |
| WIDOW | All's Well That Ends Well | Female | 64 |
| FIRST QUEEN | The Two Noble Kinsmen | Female | 63 |
| KATHERINE | Henry V | Female | 61 |
| FIRST WITCH | Macbeth | Female | 61 |
| LAVINIA | Titus Andronicus | Female | 59 |
| ANNE | Henry VIII | Female | 58 |
| DUCHESS OF GLOUCESTER | Richard II | Female | 58 |
| LADY PERCY | Henry IV Part 1 | Female | 57 |
| QUEEN ELEANOR | King John | Female | 55 |
| HOSTESS | Henry IV Part 1 | Female | 49 |
| URSULA | Much Ado About Nothing | Female | 47 |
| KATHARINE | Love's Labour's Lost | Female | 46 |
| LADY PERCY | Henry IV Part 2 | Female | 46 |
| COUNTESS | Henry VI Part 1 | Female | 45 |
| MISTRESS QUICKLY as Queen of Fairies | The Merry Wives of Windsor | Female | 44 |
| HOSTESS | Henry V | Female | 42 |
| WIFE | Macbeth | Female | 42 |
| MARIA | Love's Labour's Lost | Female | 42 |
| BLANCHE | King John | Female | 42 |
| IRIS | The Tempest | Female | 41 |
| VALERIA | Coriolanus | Female | 40 |
| SECOND QUEEN | The Two Noble Kinsmen | Female | 39 |
| HECAT | Macbeth | Female | 39 |
| THIRD QUEEN | The Two Noble Kinsmen | Female | 37 |
| CASSANDRA | Troilus and Cressida | Female | 37 |
| MISTRESS OVERDONE | Measure for Measure | Female | 36 |
| VIRGILIA | Coriolanus | Female | 36 |
| OCTAVIA | Antony and Cleopatra | Female | 36 |
| COURTESAN | The Comedy of Errors | Female | 35 |
| BIANCA | Othello | Female | 35 |
| HIPPOLYTA | A Midsummer Night's Dream | Female | 35 |
| MARGARET | Henry VI Part 1 | Female | 33 |
| ANNE | The Merry Wives of Windsor | Female | 28 |
| HELEN | Troilus and Cressida | Female | 28 |
| ALICE | Henry V | Female | 28 |
| THIRD WITCH | Macbeth | Female | 27 |
| CALPHURNIA | Julius Caesar | Female | 27 |
| SECOND WITCH | Macbeth | Female | 26 |
| IRAS | Antony and Cleopatra | Female | 26 |
| QUEEN ISABEL | Henry V | Female | 24 |
| CERES | The Tempest | Female | 24 |
| GENTLEWOMAN | Macbeth | Female | 24 |
| MOPSA | The Winter's Tale | Female | 21 |
| EMILIA | The Winter's Tale | Female | 20 |
| AUDREY | As You Like It | Female | 20 |
| MARIANA | All's Well That Ends Well | Female | 20 |
| NURSE | Titus Andronicus | Female | 19 |
| ALL WITCHES | Macbeth | Female | 19 |
| JAQUENETTA | Love's Labour's Lost | Female | 18 |
| QUEEN | King Edward III | Female | 17 |
| LADY FAULCONBRIDGE | King John | Female | 15 |
| ANDROMACHE | Troilus and Cressida | Female | 15 |
| WOMAN | The Two Noble Kinsmen | Female | 14 |
| QUEEN'S LADY | Cymbeline | Female | 13 |
| DORCAS | The Winter's Tale | Female | 13 |
| MOTHER | Cymbeline | Female | 12 |
| GENTLEWOMAN | Henry VIII | Female | 12 |
| WIDOW | The Taming of the Shrew | Female | 11 |
| LYCHORIDA | Pericles | Female | 11 |
| DIANA | Pericles | Female | 10 |
| JULIET | Measure for Measure | Female | 10 |
| FRANCISCA | Measure for Measure | Female | 9 |
| FIRST LADY | The Winter's Tale | Female | 9 |
| BONA | Henry VI Part 3 | Female | 9 |
| WOMAN | King Edward III | Female | 8 |
| WIFE | Henry VI Part 2 | Female | 8 |
| GHOST OF ANNE | Richard III | Female | 8 |
| LUCE | The Comedy of Errors | Female | 8 |
| JUNO | The Tempest | Female | 7 |
| PATIENCE | Henry VIII | Female | 6 |
| GIRL | Richard III | Female | 5 |
| LADY NORTHUMBERLAND | Henry IV Part 2 | Female | 5 |
| PHRYNIA and TIMANDRA | Timon of Athens | Female | 4 |
| TIMANDRA | Timon of Athens | Female | 4 |
| JOURDAIN | Henry VI Part 2 | Female | 4 |
| SECOND LADY | The Winter's Tale | Female | 4 |
| HOSTESS | The Taming of the Shrew | Female | 4 |
| HELENUS | Troilus and Cressida | Female | 4 |
| LADY MONTAGUE | Romeo and Juliet | Female | 3 |
| ALL QUEENS | The Two Noble Kinsmen | Female | 3 |
| FIRST LADY | Richard II | Female | 3 |
| SECOND LADY | Richard II | Female | 3 |
| DAUGHTER | Pericles | Female | 2 |
| GENTLEWOMAN | Coriolanus | Female | 2 |
| VIRGILIA and VALERIA | Coriolanus | Female | 1 |
| ALL THE LADIES | Timon of Athens | Female | 1 |
| FIRST LADY | Timon of Athens | Female | 1 |
| PHRYNIA | Timon of Athens | Female | 1 |
| NELL | The Two Noble Kinsmen | Female | 1 |
| QUEEN ELIZABETH and DUCHESS OF YORK | Richard III | Female | 1 |
| MISTRESS FORD and MISTRESS PAGE | The Merry Wives of Windsor | Female | 1 |
| WIDOW and MARIANA | All's Well That Ends Well | Female | 1 |
| FIRST LADY | Cymbeline | Female | 1 |
| LADIES | Cymbeline | Female | 1 |